Introduction
Colorectal cancer (CRC) is one of the most common digestive tract malignant tumors, and its incidence rate and mortality rate rank third among all malignant tumors. The IMMUNOGLOBULIN SUPERFAMILY DOMAINS genes has been reported to be hypermethylated in human colorectal cancer (CRC) cell lines and primary tumor tissues, .On the other hand High mobility group genes are characteristically present at high levels in a variety of human cancers including colorectal, ovarian, breast, lung, and pancreatic cancer.
Aims & Methods
The aim of the work to evaluate the gene expression of both leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) (as an example of immunoglobulin sperfamily domains and High mobility group AT-hook 2 (HMGA2) as an example of high mobility group as diagnostic and prognostic markers in cases of colorectal cancer.
In this study forty-five patients pathologically approved with colorectal cancer were prospectively analyzed for LRIG1 and HMGA2 genes expression at tissue level. SYBR Green Real-Time RT-PCR technique was used for gene expression analyses and β-actin gene was considered as a reference gene for data normalization. Additionally, appropriate statistical analyses were used to assess the expression of LRIG1 and HMGA2 in diseased and adjacent healthy tissues.
Results
HMGA2 mRNA gene expression was over-expressed in CRC tissue than normal tissue ( median fold change and median Log2 ratio are [9.3(0.2, 100.4) and 3.1(-2.6, 6.7), respectively]with positive correlation between HMGA2 and poor differentiation ,more advanced stage and lymph node metastasis(p> 0.05) but not with distant metastasis (p >0.05) , The expression of LRIG1 mRNA gene in tumor tissue was low expressed in colorectal tumor tissues (Median fold change and median Log2 ratio are [0.29(0.01, 4.14) and -1.8(-6.1, 2.1), respectively but no correlation of the LRIG1 gene with the the stage, Differentiation and metastasis of the CRC (P >0.05). There is no correlation of both genes neither with tumor biomarkers (CA19-9, CEA) nor with laboratory findings (WBCs, Hemoglobin, Platelet, INR, Albumin, Creatinine) P>0.5%). The overall survival showed no significant association with any genetic expression of both genes (P >0.05).
Conclusion
LRIG1 low expression may be considered for diagnosis but not valuable as prognostic marker in colorectal cancer patients. However, HMGA2 overexpression may be valuable as both diagnostic and prognostic markers in CRC.
Disclosure
No conflict of interest to be disclosed
