UEG's content platform for gastroenterology education bringing recordings, courses, guidelines, and more together in one place.

Re-live UEG Week 2026 on Gutflix Gut Guide online What's new on Gutflix

Trending on Gutflix

Rate for better recommendations!

Your feedback helps us show you more of what you like and less of what you dislike!

More of this!

We will show you more similar content.

Not for me!

We won't suggest this again.

Bookmark!

Save this content for later!

New to myUEG? Create an account! Log In with myUEG account

UEG Poster

Standard Poster

Share via Email Share on Facebook Share on X Share on LinkedIn Share on Bluesky

This content is part of Gutflix. Log in with your myUEG account, or create one free, to watch it.

Not sure what you can access? Learn more about account types.

ACUTE MUCOSAL REACTIONS FOLLOWING FOOD ADMINISTRATION IN IRRITABLE BOWEL SYNDROME ARE ASSOCIATED WITH MAST CELL ACTIVATION EX VIVO IN A SUBGROUP OF PATIENTS

Lukas Balsiger 1, Karlien Raymenants 1,2, Cedric Van de Bruaene 1, Jolien Schol 1, Leïla Juvyns 1, Karen Routhiaux 1, Joran Toth 1, Ricard Farré 1, Tim Vanuytsel 1,2, Jan Tack 1,2

Affiliations

1 TARGID KU Leuven, Leuven, Belgium

2 University of Leuven, University Hospital Gasthuisberg, Leuven, Belgium

Summary

AI Generated

Summary is not available for this content yet.

Download PDF

Was this helpful?

Thanks for your feedback.

This summary was generated by an AI large language model based on the content transcript. It is for informational purposes only and should not be considered a substitute for clinical judgment. Always rely on your professional expertise and the full clinical context when making clinical decisions.

Abstract

Introduction

Irritable bowel syndrome (IBS) is a prevalent disorder of gut-brain interaction characterized by abdominal pain and altered bowel movements. While the pathophysiology underlying IBS is incompletely understood, local allergic reactions to foods are a candidate mechanism. Confocal laser endomicroscopy (CLE) has shown acute alterations of the duodenal epithelium in IBS within minutes of local food administration and these findings have been used to identify foods contributing to IBS symptoms. However, the mechanisms and relevance of the observed alterations remain elusive.

Aims & Methods

Aim: To visualize the effect of local food administration on the duodenal mucosa using CLE and assess whether visible changes are associated with altered permeability or markers of mast cell activation ex vivo.
Methods: Non-constipated Rome IV IBS patients with negative serum specific IgE for tested foods underwent baseline gastroscopy (EGD) and CLE performed during a second EGD. Duodenal mucosa was visualized using CLE at baseline and after luminal administration of foods (egg white, soy, milk, wheat, lyophilized fish and shellfish, and nuts solutions sequentially applied in a random order) through the working channel. Altered mucosa was defined as leakage of fluorescein into the lumen and presence of particles (previously termed “cell shedding”). Serum samples were taken at index endoscopy and 30 minutes after acute mucosal alterations. Biopsies were taken at index EGD and after visualized alterations to measure transepithelial electrical resistance (TEER) in Ussing chambers and tryptase levels in biopsy supernatants. Data are reported as mean ± SEM or median and IQR as appropriate.

Results

45 CLE exams were carried out in 28 patients (age 35±2 y, 93%f, BMI 24±1 kg/m2, mean IBS symptom severity score 320±20).
At baseline, mucosal alterations were present in 9% of exams (n=4), whereas all other exams (n=41) demonstrated alterations after food administration. The highest rate of acute alterations was observed after fish/shellfish mix (50%; 10 altered / 20 exposed). The rate of observed alterations per food is presented in the table. TEER was not altered after observed alterations vs baseline (25±0.8 Ωxcm² vs 25±1 Ωxcm²). Serum tryptase was not altered after food exposure vs baseline (ratio after/before = 0.9, IQR= 0.8-1.1, n=13). Tryptase release from biopsies increased after exposure compared to baseline in 5/9 (56%) of patients after one hour and in 13/19 (68%) patients after 24h. Overall, the average tryptase release of the tissue was 1.48-fold increased after food exposure compared to baseline in the first hour after exposure (IQR = 0.3-2.1, n=9) and 1.29-fold following 24h incubation (IQR = 0.9-2.1, n = 19).

Food	Exposed	Altered n(%)	Food	Exposed	Altered n(%)
Fish/Shellfish mix	20	10 (50%)	Egg	21	6 (29%)
Milk	21	10 (48%)	Wheat	18	5 (28%)
Nuts	19	6 (32%)	Soy	15	4 (27%)

Conclusion

In this group of IBS patients, we found a high rate of acute food-induced mucosal alterations as visualized with CLE. Ex vivo permeability was unaltered after observed alterations vs. baseline. Systemic tryptase remained unchanged after acute alterations compared to baseline whereas tryptase release from biopsies was increased following food exposure compared to baseline in more than half of patients.

Conference

UEG Week Vienna 2024

Topics

Colorectal

Submission format

Abstract

Session

Potpourri of DGBI and motility (Posters)

Citation

United European Gastroenterology Journal 2024; 12 (Supplement 8)

Published

2024

More Like This:

A DECADE OF RISING REFLUX: THE ESCALATING BURDEN OF GASTROESOPHAGEAL REFLUX DISEASE (GERD) IN EUROPE (2010–2021) AMID GLOBAL TRENDS

AHMAD SANAN

GUT HEALTH AND LIPID METABOLISM: THE ROLE OF SIBO THERAPY

Volodymyr Vozniuk

UPADACITINIB FOR REFRACTORY COELIAC DISEASE AND CONCOMITANT MICROSCOPIC COLITIS

Arvind Rajagopalan

PROTECTIVE EFFECTS OF STW 5 AGAINST CHANGES IN THE MICROBIOME IN AN EXPERIMENTAL MODEL OF FUNCTIONAL DYSPEPSIA

Aziz-Kalbhenn Heba

ESOPHAGEAL FUNCTION TESTING IDENTIFIES ASYMPTOMATIC BARRETT’S ESOPHAGUS PATIENTS WITH PATHOLOGICAL ACID EXPOSURE AT RISK FOR NEOPLASIA: A MULTICENTER STUDY

Elisa Marabotto

FROM INTOXICATION TO ENCEPHALOPATHY: THE CASCADE OF ALCOHOL-INDUCED LIVER INJURY AND MULTIORGAN DYSFUNCTION IN A YOUNG WOMAN

Gagik Hakobyan

UEG Presentation

Share via Email Share on Facebook Share on X Share on LinkedIn Share on Bluesky

A DECADE OF RISING REFLUX: THE ESCALATING BURDEN OF GASTROESOPHAGEAL REFLUX DISEASE (GERD) IN EUROPE (2010–2021) AMID GLOBAL TRENDS

This content is part of Gutflix. Log in with your myUEG account, or create one free, to watch it.

Not sure what you can access? Learn more about account types.